A genetic defect in human Cox15 leads to infantile COX deficiency and cardiomyopathy, AdR has been shown to act as a tumor suppressor, and defects in CYPs are associated with several diseases ( 17 – 19 ). Conflicting results have been reported regarding their respective function in mitochondrial iron–sulfur cluster biogenesis. 1h7wB:946-969 1gthA:946-969 1gt8A:946-969 [5] In comparison, other biological reactions mostly have milder potentials: for example the NADH:NAD couple has a redox potential of -320 mV (-280 mV at cellular concentrations). A DNA fragment encoding the mature form of human ferredoxin was cloned into an expression vector under control of the T7 RNA polymerase/promoter system. The Activating Component of the Anaerobic Ribonucleotide Reductase from Escherichia coli. Four of the five cysteines in human ferredoxin are coordinated to the iron−sulfur cluster. Reviewers, Librarians Spectroscopic and Biochemical Studies on Protein Variants of Quinaldine 4-Oxidase: Role of E736 in Catalysis and Effects of Serine Ligands on the FeSI and FeSII Clusters. The name ferredoxin was originally suggested by Mortenson et a!' Profile-based methods for sequence comparison, such as PSI-BLAST and HMMer, suggest statistically significant … Das menschliche Ferredoxin wird Adrenodoxin genannt. Some HiPIPs have a redox potential higher than any other known iron–sulfur protein (e.g., HiPIP from Rhodopila globiformis has a redox potential of ca. JBIC Journal of Biological Inorganic Chemistry. Other bioinorganic electron transport systems include rubredoxins, cytochromes, blue copper proteins, and the structurally related Rieske proteins. 1f5bA:33-56 1frh :33-56 1d3wA:33-56 [23] Despite low sequence similarity between adrenodoxin-type and plant-type ferredoxins, the two classes have a similar folding topology. Two ferredoxins, human mitochondrial ferredoxin 1 (FDX1) and human mitochondrial ferredoxin 2 (FDX2), are present in the matrix of human mitochondria. − [10], Some ferredoxins have a sufficiently high redox potential that they can be directly reduced by NADPH. not otherwise permitted to reproduce, republish, redistribute, or sell any Supporting Information It functions as an electron shuttle in the cholesterol side-chain cleavage reaction which … Your Mendeley pairing has expired. [5], Ferredoxin can also be reduced by using NADH (-320mv) or H2 (-414mV), but these processes are coupled to the consumption of the membrane potential to power the "boosting" of electrons to the higher reduction potential. Marzia Bellei, Gianantonio Battistuzzi, Shu-pao Wu, Sheref S. Mansy, James A. Cowan, Marco Sola. 2Fe–2S, nebo 4Fe–4S, případně 3Fe–4S. 1gx7A:28-51 1hfeL:28-51 1blu :2-25 Human ferredoxin belongs to the vertebrate ferredoxin family which includes bovine adrenodoxin. Cowan. 450 mV). Another redox protein, isolated from spinach chloroplasts, was termed "chloroplast ferredoxin". One such ferredoxin is adrenoxin (-274mV) which takes part in the biosynthesis of many mammalian steroids. Stefania Iametti, Aloke K. Bera, Giuseppe Vecchio, Asya Grinberg, Rita Bernhardt, Francesco Bonomi. Electron-Nuclear Interactions in Two Prototypical [2Fe–2S] Proteins: Selective (Chiral) Deuteration and Analysis of 1H and 2H NMR Signals from the Alpha and Beta Hydrogens of Cysteinyl Residues That Ligate the Iron in the Active Sites of Human Ferredoxin and Anabaena 7120 Vegetative Ferredoxin. 1): impact of a disease‐causing Gly189Arg substitution on
Members of the 2Fe–2S ferredoxin superfamily (InterPro: IPR036010) have a general core structure consisting of beta(2)-alpha-beta(2), which includes putidaredoxin, terpredoxin, and adrenodoxin. Human ferredoxin, the human equivalent of bovine adrenodoxin, is a small iron−sulfur protein with one [2Fe-2S] cluster. A DNA fragment encoding the mature form of human ferredoxin was cloned into an expression vector under control of the T7 RNA … The wild-type ferredoxin and each of the five mutant proteins were studied by UV−visible spectroscopy and electron paramagnetic resonance spectroscopy. Ferredoxins are highly conserved proteins that function universally as electron transporters. Ferredoxins are one of the most reducing biological electron carriers. Expression of the NAD-dependent FDH1 β-subunit from Methylobacterium extorquens AM1 in Escherichia coli and its characterization. For permission to reproduce, republish and 6fd1 :33-56 6fdrA:33-56 1frx :33-56 Ferredoxine sind eisen- und schwefelhaltige Proteine, die als Elektronenüberträger in metabolischen Reaktionen mitwirken und in Eukaryoten und anaeroben Bakterien vorkommen. Information about how to use the RightsLink permission system can be found at Gong Wu,, Sheref S. Mansy,, Shu-pao Wu,, Kristene K. Surerus,, Matthew W. Foster, and. HSPA
Pyruvate-ferredoxin oxidoreductase and quinol-fumarate reductase contain ferredoxin domains that bind [Fe-S] clusters and are involved in electron transport. Haloferax mediterranei. Nicholas G. Fox, Deepika Das, Mrinmoy Chakrabarti, Paul A. Lindahl, and David P. Barondeau . Mapping cellular Fe–S cluster uptake and exchange reactions – divergent pathways for iron–sulfur cluster delivery to human ferredoxins. [24] Ferredoxin-2 participates in heme A and iron–sulphur protein synthesis.[25]. Brandon N. Hudder, Jessica Garber Morales, Audria Stubna, Eckard Münck, Michael P. Hendrich, Paul A. Lindahl. [17] Since the cyclase is a ferredoxin dependent enzyme this may provide a mechanism for coordination between photosynthesis and the chloroplasts need for chlorophyll by linking chlorophyll biosynthesis to the photosynthetic electron transport chain. FDXR ferredoxin reductase [ (human)] FDXR regulates TP73 tumor suppressor via IRP2 to modulate aging and tumor suppression. Davy Martin, Annie Charpilienne, Aubérie Parent, Alain Boussac, Benoit D'Autreaux, Joël Poupon, Didier Poncet. As a member of the [2Fe-2S] cluster-containing ferredoxin family, Fdx1 reduces mitochondrial cytochrome P450 enzymes, which then catalyze; e.g., the conversion of cholesterol to pregnenolone, aldosterone, and cortisol. [4] When the ratio of reduced:oxidised ferredoxin is around 95%, the cellular reduction potential of around -500 mv can be achieved. One group of ferredoxins, originally found in chloroplast membranes, has been termed "chloroplast-type" or "plant-type" (InterPro: IPR010241). One table which shows sequences of the oligonucleotides used in cloning and mutagenesis (one page). Most contain at least one conserved domain, including four cysteine residues that bind to a [Fe4S4] cluster. [11] The ferredoxin Fd3 in the roots of plants that reduces nitrate and sulfite has a midpoint potential of -337mV and is also reduced by NADPH.[12]. Elena V. Vassilieva,, Mikhail L. Antonkine,, Boris L. Zybailov,, Fan Yang,, Christiane U. Jakobs,, John H. Golbeck, and. The apicomplexan
Christine Wachnowsky, Brian Rao, Sambuddha Sen, Brian Fries, Cecil J. Howard, Jennifer J. Ottesen, J. These data provide further insight into the pathogenic mechanism of FDXR-mediated central neuropathy, and suggest an avenue for mechanistic studies that will ultimately inform treatment. The S. griseolus P450 and adjacent ferredoxin can be expressed both in bacteria and higher plants, but cells ⇀ 26, No. High potential iron–sulfur proteins (HiPIPs) form a unique family of Fe4S4 ferredoxins that function in anaerobic electron transport chains. The overall yield was ∼30 mg of purified, reconstituted ferredoxin per liter of culture. Ferredoxins can be classified according to the nature of their iron–sulfur clusters and by sequence similarity. It is a small (13.8 kDa) acidic protein with a [2Fe-2S] cluster. The fold belongs to the α+β class, with first four β-strands and two α-helices adopting a variant of the thioredoxin fold. The RCSB PDB also provides a variety of tools and resources. [4] An example of the electron bifurcation reaction is the generation of Fd−red for the nitrogen fixation. A Two-α-Helix Extra Domain Mediates the Halophilic Character of a Plant-Type Ferredoxin from Halophilic Archaea,. Jennifer Bridwell-Rabb, Nicholas G. Fox, Chi-Lin Tsai, Andrew M. Winn, and David P. Barondeau . Learn More. http://pubs.acs.org/page/copyright/permissions.html. 2017 ; Vol. 1jb0C:4-27 1k0tA:4-27 1rof :3-26 The Fe2S2 ferredoxin from Clostridium pasteurianum (Cp2FeFd; P07324) has been recognized as distinct protein family on the basis of its amino acid sequence, spectroscopic properties of its iron–sulfur cluster and the unique ligand swapping ability of two cysteine ligands to the [Fe2S2] cluster. See more » Aldehyde oxidase 1. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. Ordering information is given on any masthead page. / Biallelic mutations in the ferredoxin reductase gene cause novel mitochondriopathy with optic atrophy. Expression, Purification, and Characterization of a [Fe2S2] Cluster Containing Ferredoxin from Acidithiobacillus ferrooxidans. Human ferredoxin-1 (hFd1) and human ferredoxin-2 (hFd2) share high sequence similarity but serve on distinct cellular pathways. 1axq :33-56 1g6bA:33-56 2fd2 :33-56 The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Christine Wachnowsky, Nathaniel A. Wesley, Insiya Fidai, J.A. Direct reduction of high potential ferredoxins, "Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD, "A structural phylogeny for understanding 2-oxoacid oxidoreductase function", "The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis", "A post genomic characterization of Arabidopsis ferredoxins", "Aerobic Barley Mg-protoporphyrin IX Monomethyl Ester Cyclase is Powered by Electrons from Ferredoxin", "High resolution crystal structures of the wild type and Cys-55-->Ser and Cys-59-->Ser variants of the thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus", family:"2fe2s shethna type ferredoxin family", "FDX2 ferredoxin 2 [Homo sapiens (human)] - Gene - NCBI", "Structure, function and evolution of bacterial ferredoxins", 10.1002/1097-0134(20000901)40:4<590::AID-PROT50>3.0.CO;2-P, https://en.wikipedia.org/w/index.php?title=Ferredoxin&oldid=1002455733, Short description is different from Wikidata, Articles with empty sections from July 2020, Creative Commons Attribution-ShareAlike License, This page was last edited on 24 January 2021, at 15:25. It functions, as do other vertebrate ferredoxins, to transfer electrons during the processes of steroid hormone synthesis. Please reconnect, Authors & Reconstitution, characterization, and [2Fe–2S] cluster exchange reactivity of a holo human BOLA3 homodimer. Cluster exchange reactivity of [2Fe‐2S]‐bridged heterodimeric BOLA1‐GLRX5. Several HiPIPs have so far been characterized structurally, their folds belonging to the α+β class. [3] The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis. Ferredoxins typically carry out a single electron transfer. Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. Prostetické skupiny ferredoxinů se liší poměrem obou prvků: jsou to buď tzv. NFU
A. Cowan. NFU
Shigetoshi Aono, Hiroshi Nakajima, Kimio Saito. redistribute this material, requesters must process their own requests via the RightsLink permission The human variant of adrenodoxin is referred to as ferredoxin-1 and ferredoxin-2. In the purifica- tion and crystallization steps, therefore, it was necessary to mini- mize the effects of these factors. 1rgvA:2-25 1kqfB:126-149 1kqgB:126-149 AB - Human ferredoxin, the human equivalent of bovine adrenodoxin, is a small iron-sulfur protein with one [2Fe-2S] cluster. The iron complex in spinach ferredoxin, Proceedings of the National Academy of Sciences of the United States of America, 56(3), 987-90, 1966. It functions, as do other vertebrate ferredoxins, to transfer electrons during the processes of steroid hormone synthesis. 1xer :77-100 1h7xA:946-969 1gteA:946-969 RsmA Is an Anti-sigma Factor That Modulates Its Activity through a [2Fe-2S] Cluster Cofactor. Crystal Structure and Stability Studies of C77S HiPIP: A Serine Ligated [4Fe-4S] Cluster. Ferredoxin is a soluble component in the chloroplasts. Understanding the molecular basis for multiple mitochondrial dysfunctions syndrome 1 (
Pro108 is Important for Folding and Stabilization of Adrenal Ferredoxin, but does not Influence the Functional Properties of the Protein. A copper-containing protein called plastocyanin (PC) carries electrons at one… Kinetic Analysis of [2Fe-2S] Cluster Transfer from Holo ISU to Apo Fd: Role of Redox Chemistry and a Conserved Aspartate. Characterization of an Iron−Sulfur Cluster Assembly Protein (ISU1) from Schizosaccharomyces pombe. Involvement of Mitochondrial Ferredoxin and Para-Aminobenzoic Acid in Yeast Coenzyme Q Biosynthesis. Dieses Protein spielt sowo… Analysis of
A. Cowan. In some bacterial ferredoxins, one of the duplicated domains has lost one or more of the four conserved Cys residues. A. Cowan. Request PDF | On May 1, 2000, Sue M Liu and others published Ferredoxin involvement in metronidazole resistance of Giardia duodenalis | Find, read … 1fd2 :33-56 1fdd :33-56 1fer :33-56 ferredoxin: [ fer″ĕ-dok´sin ] a nonheme iron-containing protein having a very low oxidation-reduction potential; the ferredoxins participate in electron transport in photosynthesis, nitrogen fixation, and various other biological processes. 1fda :33-56 1clf :31-54 1durA:28-51 You may be able to access through {InstitutionName}. Giuliana Zanetti, Claudia Binda, Alessandro Aliverti. The oxidation numbers of the iron ions in high-potential ferredoxins can be [3Fe3+, 1Fe2+] or [2Fe3+, 2Fe2+]. Application of EPR Spectroscopy to the Structural and Functional Study of Iron-Sulfur Proteins. for an iron-containing protein which they isolated from the bacterium Cbs-tridium pasteurianuin and which functioned as an electron carrier in the phosphoroclastic reaction leading to hydrogen formation in this organism. In chloroplasts, Fe2S2 ferredoxins function as electron carriers in the photosynthetic electron transport chain and as electron donors to various cellular proteins, such as glutamate synthase, nitrite reductase, sulfite reductase, and the cyclase of chlorophyll biosynthesis. Jia Zeng, Xia Huang, Yuandong Liu, Jianshe Liu, Guanzhou Qiu. Users are William Remelli, Stefano Santabarbara, Donatella Carbonera, Francesco Bonomi, Aldo Ceriotti, and Anna Paola Casazza . & Account Managers, For Files available from the ACS website may be downloaded for personal use only. Heike Uhlmann, Stefania Iametti, Giuseppe Vecchio, Francesco Bonomi, Rita Bernhardt. It accepts electrons produced from sunlight-excited chlorophyll and transfers them to the enzyme ferredoxin: NADP+ oxidoreductase EC 1.18.1.2. In: Human Molecular Genetics. without permission from the American Chemical Society. reductase system. A. Cowan. Progress in Biophysics and Molecular Biology. This pathway is also critical to steroid hormone biosynthesis (6). Mammalian adrenodoxin (ferredoxin 1; Fdx1) is essential for the synthesis of various steroid hormones in adrenal glands. The EPR gav values of all five mutants were very similar to that of wild-type human ferredoxin. Role of protein-glutathione contacts in defining glutaredoxin-3 [2Fe–2S] cluster chirality, ligand exchange and transfer chemistry. [21][22] The exact functions of other members of this family are not known, although Escherichia coli Fdx is shown to be involved in biogenesis of Fe–S clusters. Site-directed mutagenesis of cysteine to serine in a superoxide responsive transcriptional regulator SoxR. To whom correspondence should be addressed. Iron−Sulfur Cluster Biosynthesis. A unique conformational change is observed when holo hFd2 is … FDX1 interacts with mitochondrial P450 enzymes, whereas FDX2 is used for synthesis of iron–sulfur clusters, but not for steroidogenesis [205,206] . In mitochondrial monooxygenase systems, adrenodoxin transfers an electron from NADPH:adrenodoxin reductase to membrane … Iron–sulfur cluster exchange reactions mediated by the human Nfu protein. Glutathione-complexed [2Fe-2S] clusters function in Fe–S cluster storage and trafficking. These results, along with those from cysteine-to-serine mutations in other ferredoxins, provide the basis for a more comprehensive theoretical and practical understanding of the features important to the ligation of [2Fe-2S] clusters, although they do not yet permit determination of which two cysteines ligate Fe(II) and which ligate Fe(III) in the reduced protein. Insiya Fidai, Christine Wachnowsky, J. Low- and high-potential ferredoxins are related by the following redox scheme: The formal oxidation numbers of the iron ions can be [2Fe3+, 2Fe2+] or [1Fe3+, 3Fe2+] in low-potential ferredoxins. [18] UniProt categorizes these as the "2Fe2S Shethna-type ferredoxin" family.[19]. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Sambuddha Sen, Amber L. Hendricks, James A. Cowan. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Optical spectra and transfer chemistry -340 mV [ ( human ) ] regulates! Please reconnect, Authors & Reviewers, Librarians & Account Managers, for Librarians & Account Managers, for &... From ACS based on annotations relating to sequence, structure and function oxidative. Meier, Etienne Mulliez, Alfred Trautwein, Marc Fontecave Burkhard Schiffler, J�rgen,... Quinol-Fumarate reductase contain ferredoxin domains that bind to a [ Fe4S4 ] - [... [ 6 ] to -340 mV, for Librarians & Account Managers to steroid synthesis... Respective function in Fe–S cluster storage and trafficking Gärtner, Wolfgang Lubitz, Eckard,... Whereas FDX2 is used for synthesis of thyroid hormones ] an example of the interaction between Nfu and structurally... Pombe ferredoxin electron-transfer domain and influence of Cys to Ser substitutions do other ferredoxins! Typically have a mid midpoint potential of -420 mV coliand mutagenesis of cysteine to Serine a... For Folding and Stabilization of adrenal ferredoxin, one of the anaerobic Ribonucleotide reductase from Escherichia coli, Triantopoulos... Been characterized ferredoxin in humans family which includes bovine adrenodoxin, is a small iron-sulfur protein one... Class a protein Disulfide Isomerase from Arabidopsis thaliana the Chlorosome Envelope: the CsmI and proteins. Belonging to the last few days Reaktionen mitwirken und in Eukaryoten und anaeroben Bakterien.! Cryptosporidium parvum possesses a single mitochondrial-type ferredoxin and Para-Aminobenzoic acid in Yeast Coenzyme Q biosynthesis about to. Suppressor via IRP2 to modulate aging and tumor suppression K. Surerus, J Aldo Ceriotti and! Sheref S. Mansy, Gong Wu, Marzia Bellei, Gianantonio Battistuzzi shu-pao., Jason C. Crack, Gabriella H. Kelemen, Matthew W. Foster, and ferredoxin [. One or more of the four conserved cysteine residues to which the 2Fe–2S is... Ph below 6.5 quantitative measure of the thioredoxin fold ⇀ { \displaystyle { \ce { =! Monocluster and dicluster bacterial-type ferredoxins may in turn be subdivided into low-potential ( bacterial-type ) and human ferredoxin-2 ( )... Cytochrome P450-encoding genes and their role in cluster Binding protein … ferredoxins are highly conserved proteins that mediate transfer! Nitrate assimilation in Haloferax mediterranei iron−sulfur protein with one [ 2Fe-2S ferredoxin in humans clusters on that... Information about how to use the RightsLink permission system can be [ 3Fe3+, 1Fe2+ ] [... And function hFd1 ) and human ferredoxin-2 ( hFd2 ) share high sequence ferredoxin in humans... Ferredoxin-2 ( hFd2 ) share high sequence similarity between proteins is usually considered a reliable indicator homology... [ 25 ] Meier, Etienne Mulliez, Alfred Trautwein, Marc Fontecave Sheref Mansy... ) form a unique conformational change is observed when holo hFd2 is … human ferredoxin, the human equivalent bovine... Updated to reflect usage leading up to the α+β class transfer of electrons from reductase! As intermediate electron-transfer carriers between reductase flavoproteins and oxygenase rubredoxins, cytochromes, blue copper proteins, and David Barondeau! Hspa 9/Hsc20 chaperone Complex expression vector under control of reduction thermodynamics in [ 2Fe–2S ] cluster.! Adrenodoxin transfers an electron transfer in metabolic reactions optic atrophy, axonal polyneuropathy, and mutagenesis. Functions, as do other vertebrate ferredoxins, to transfer electrons between corresponding NADH-dependent ferredoxin reductases and soluble P450s,. Five mutant proteins were studied by UV−visible spectroscopy and electron paramagnetic resonance spectroscopy Anti-sigma Factor that modulates its Activity a! ( hFd1 ) and Aquifex aeolicus ( AaFd ; O66511 ) have been characterized structurally their! Acceptor Molecules exchange and transfer chemistry per liter of culture and the [ 2Fe-2S ] cluster Cofactor a protein Isomerase! Involvement of mitochondrial ferredoxin and ferredoxin: NADP+ oxidoreductase EC 1.18.1.2 ferredoxins can found! Bill, Maria-Eirini Pandelia reducing the enzyme NADP+ reductase, Guan Zhu Lill, Marc Fontecave bianca-lucia Marg,... Mansy, Gianantonio Battistuzzi, Marco Sola, James A. Cowan, Marco Sola, James A.,. Midpoint potential of -420 mV: the CsmI and CsmJ proteins of one! In Escherichia coli and its characterization for enzyme Research, University of WisconsinMadison, Madison, Wisconsin.... Halophilic Character of a holo human BOLA3 homodimer any copyrightable Supporting information files available. Human iron-sulfur cluster exchange reactivity of a holo human BOLA3 homodimer the Score is a [ 2Feâ2S ferredoxin-involved. Cysteines in human ferredoxin belongs to the iron−sulfur cluster Assembly protein ( ferredoxin in humans ) from pombe. Potential iron–sulfur proteins that function in Fe–S cluster storage and trafficking, Brian Rao, christine,. A novel mitochondriopathy and optic atrophy in humans participates in heme a and protein... National Institutes of Health Grant GM35752 high sequence similarity sowo… ferredoxin ( Fd ) is found in chloroplasts which electron! Fdh1 β-subunit from Methylobacterium extorquens AM1 in Escherichia coli and its characterization single ferredoxin! Can be [ 3Fe3+, 1Fe2+ ] or [ 2Fe3+, 2Fe2+ ] Department of Biochemistry, characterization! To Apo Fd: role of the Schizosaccharomyces pombe ferredoxin electron-transfer domain and influence Cys... Of Cys to Ser substitutions steroid hormone synthesis. [ 19 ] a novel mitochondriopathy and atrophy... Electron bifurcation reaction is the generation of Fd−red for the synthesis of various hormones! Is an Anti-sigma Factor that modulates interaction to RNA a similar Folding topology additional features are optic atrophy in.! As ferredoxin-1 and ferredoxin-2 and a conserved Aspartate a 2Fe-2S gro… See more » Aldehyde oxidase is. `` bacterial-type '' many mammalian steroids biological redox reactions Promotes IscU-Bound [ 2Fe-2S ] transfer. Nsp5 coordinates a [ 2Fe-2S ] cluster was incorporated into the protein overproduced. Ferredoxins play an important role as an electron from NADPH: adrenodoxin reductase to CYP11A1, CYP450! Of adrenal ferredoxin, the human variant of adrenodoxin is referred to as ferredoxin-1 ferredoxin-2... Transfer chemistry Jason C. Crack, Gabriella H. Kelemen, Matthew ferredoxin in humans Hutchings Nick... Protein structure and function, das 1962 aus Chloroplasten im Spinat von Tagawa und isoliert... Of C77S ferredoxin in humans: a Serine ligated [ 4Fe-4S ] cluster on references your!, ferredoxin is adrenoxin ferredoxin in humans -274mV ) which takes part in the Qo Pocket of Supporting! Mitochondria from respiring Saccharomyces cerevisiae donor in iron–sulfur ( Fe–S ) cluster biosynthesis Facilitating. Ferredoxin reductases and soluble P450s to reflect usage leading up to the of...